WebThermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The … WebPlasmid pCA24N-ligase from Dr. Wayne Patrick's lab contains the insert T4 DNA ligase and is published in Protein Eng Des Sel. 2013 Jul;26(7):471-8. doi: 10.1093/protein/gzt024. Epub 2013 Jun 10. This plasmid is available through Addgene. ... Engineered DNA ligases with improved activities in vitro. Wilson RH, Morton SK, Deiderick H, Gerth ML ...
Golden Gate Assembly - Bennett Lab Wiki - Rice University
Webduplex DNA, RNA or DNA/RNA hybrids (1). Source of Protein A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene. Supplied in 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% glycerol pH 7.5 @ 25°C Supplied with B1010 (2X Rapid Ligation Buffer) B6030 (10X T4 DNA Ligase Buffer) 2X Rapid Ligation Buffer (B1010): 132mM … WebT4 DNA Ligase MBG For joining both blunt-ended and cohesive-ended restriction fragments of DNA UDGase For the catalyzation of the release of uracil from uracil-containing single-stranded or double-stranded DNA Tth DNA Ligase For ligase chain reaction and ligase detection reaction Taq DNA Ligase members of the challenger crew
Addgene: pCA24N-ligase
WebT7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It will catalyze the formation of a phosphodiester bond between adjacent 5´ phosphate and 3´ hydroxyl groups of duplex DNA. Cohesive end … WebAug 30, 2013 · Ligation Protocol with T7 DNA Ligase (M0318) Set up the following reaction in a microcentrifuge tube on ice. (T7 DNA Ligase should be added last. Note that the … WebT4 DNA ligase and hLig3 exhibit more uniform ligation bias compared to T7 DNA ligase. GC-rich overhangs tend to ligate more efficiently compared to ATrich overhangs. Each overhang is... members of the cast